ISSN 1002-1027  CN 11-2952/G2

Acta scientiarum naturalium Universitatis Pekinensis

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DNA Ioslation and Sequence Analysis of Dinosaur DNA from Cretaceous Dinosaur Egg in Xixia Henan, China

LI Yi, AN Chengcai, ZHU Yuxian, ZHANG Yun, LIU Yifei, QU Lin, YOU Lingtao, LIANG Xiaowen, LI Xiaohua, QU Lijia, ZHOU Zengquan, CHEN Zhangliang   

  1. College of Life Sciences, Peking University, Beijing, 100871
  • Received:1995-02-05 Online:1995-03-20 Published:1995-03-20

Abstract: DNA was extracted from 70-80 million year old dinosaur egg excavated from Cretaceous belt formation in Xixia Henan, China. The DNA samples from both outer shell and inner floccule contents were used as templates in polymerase chain reactions (PCR) with a specific primer andhexamers. With these two primers a 150bp DNA fragment has been amplified, cloned and sequenced. Deduced amino acid sequence of this dinosaur DNA hasbeen compared with known protein sequences in EMBL Bank. The results showedthat it has significant homology with Xenopus laevis epithelia precuraor-EP-cadherin (40.6%) and brain-cadherin (37.5%), bovine placental cadherin (34.4%) and human placental cadherin (36.4%), respectively.