ISSN 1002-1027  CN 11-2952/G2

Acta scientiarum naturalium Universitatis Pekinensis

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Primary Study on Gene Expression during Root Differentiation from Rice Calli

GUO Lei1, CHENG Yinghao1, WANG Ziyi1, HE Bin1, ZHANG Junjun1, LIU Shiqing1, LIU Meihua1, CHEN Zhangliang1, 2, QU Lijia1, 2, GU Hongya1, 2, 3   

  • Received:2005-06-17 Online:2006-03-20 Published:2006-03-20

Abstract: Rice calli keep proliferating constantly without differentiation on N6 medium, but can be induced to differentiate to form roots when they are treated with ABA. Basing on this physiological feature, RNAs were extracted from the calli cultured on mediums with or without ABA respectively, and hybridized with the rice cDNA microarray. By analyzing the cDNA microarray data, The authors totally obtained 271 genes associated with root differentiation from calli, including 107 up-regulated genes and 164 down-regulated genes. This preliminary result provides new clues for further study on the molecular mechanism of root differentiation of rice calli.