Acta scientiarum naturalium Universitatis Pekinensis
QIN Yan, CUI Honglian, HU Meihao
The L1 major capsid protein of human papillomavirus type 16 was expressed in sf9 insect cells by recombinant baculoviruses and self-assembled into virus-like particles (VLPs). The VLPs were harvested by CsCl ultracentrifugation and were disassembled with DTT and EDTA. A reassembled process was performed by removing DTT and EDTA in the presence of E6E7-pcDNA3.1+ plasmid and Ca2+. The novel VLPs, in which the plasmid was packed, were named HPV16 pseudovirus.
QIN Yan,CUI Honglian,HU Meihao. In Vitro Construction of Pseudovirions of Human Papillomavirus Type 16[J].Acta scientiarum naturalium Universitatis Pekinensis, 2006, 42(2): 180-183.
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