ISSN 1002-1027  CN 11-2952/G2

Acta scientiarum naturalium Universitatis Pekinensis

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In Vitro Construction of Pseudovirions of Human Papillomavirus Type 16

QIN Yan, CUI Honglian, HU Meihao   

  1. College of life sciences, Peking University, Beijing, 100871
  • Received:2005-06-17 Online:2006-03-20 Published:2006-03-20

Abstract: The L1 major capsid protein of human papillomavirus type 16 was expressed in sf9 insect cells by recombinant baculoviruses and self-assembled into virus-like particles (VLPs). The VLPs were harvested by CsCl ultracentrifugation and were disassembled with DTT and EDTA. A reassembled process was performed by removing DTT and EDTA in the presence of E6E7-pcDNA3.1+ plasmid and Ca2+. The novel VLPs, in which the plasmid was packed, were named HPV16 pseudovirus.