ISSN 1002-1027  CN 11-2952/G2

Acta scientiarum naturalium Universitatis Pekinensis

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Preparation and Characteristics of Immobilized Laccase from Coriolus versicolor on Chitosan

CHEN Hui1, ZHANG Jianbo1, 3, WANG Weijing1, YANG Yuxiang2   

  • Received:2005-02-23 Online:2006-03-20 Published:2006-03-20

Abstract: Laccase has been immobilized on chitosan with glutaraldehyde as crosslinking agent. The effects on the activity of immobilized laccase, such as concentration of glutaraldehyde, crosslinking time, laccase dosage and reaction time were studied. The results showed the optimal conditions of immobilization: the optimal glutaraldehyde concentration was 5%, the optimal crosslinking time was 8h, the optimal laccase dosage was 20mg/g carrier, and the optimal reaction time was 6h. On this condition, the recovery of enzyme activity was 52.2%. The results indicated that the optimal pH for immobilized laccase was around 4.5, which is lower than the best pH 5.0 for free enzyme. Compared with the free enzyme, its stability and repetition were considerably improved.